Fig. 6: PDK4 function on decidualization.
From: Aldosterone from endometrial glands is benefit for human decidualization
a PDK4 mRNA level in decidual cells transfected with control siRNA (NC) or PDK4 siRNA. b Western blot analysis of PDK4 and p-PDHE1α protein in decidual cells transfected with PDK4 siRNA. c IGFBP1 mRNA level after transfection with PDK4 siRNA. d PRL mRNA level after transfection with PDK4 siRNA. e FOXO1 mRNA level after transfection with PDK4 siRNA. f Western blot analysis of FOXO1 and p-CREB proteins in the nuclear fractions and CREB protein in decidual cells after transfection with PDK4 siRNA. g Effects of DCA on IGFBP1 mRNA level. h Effects of DCA on PRL mRNA level. i Effects of DCA on FOXO1 mRNA level. j Western blot analysis of p-PDHE1α, FOXO1 (nucleus), CREB, and p-CREB (nucleus) protein expression after decidual cells were treated with DCA. k The co-immunoprecipitation of PDK4 by p-CREB antibody in decidual cells. RPL7 was used as internal control for real time PCR, GAPDH as internal control and LAMIN A/C as nuclear control for western blot. Dichloroacetate (DCA), an inhibitor of all four PDK isoforms.
