Fig. 6: IR regulates ALX/FPR2 expression. | Cell Death & Disease

Fig. 6: IR regulates ALX/FPR2 expression.

From: LXA4-FPR2 signaling regulates radiation-induced pulmonary fibrosis via crosstalk with TGF-β/Smad signaling

Fig. 6

a Representative image (top) and quantification (bottom) of irradiated lung tissues (75 Gy) stained for FPR2 and α-SMA at the indicated times. Magnification, ×1.25 and ×40. b FPR2 mRNA levels in IR-exposed mouse lung tissue measured using quantitative real-time PCR. c FPR2 expression measured by western blotting after exposure of MLE12 cells and HUVECs to 6 Gy IR. d FPR2 expression 24 h after 3 and 6 Gy IR in MLE12 cells and HUVECs; FPR2 (green); DAPI (blue). e FPR2 mRNA levels in MLE12 cells and HUVECs exposed to 6 Gy IR, measured using quantitative real-time PCR. f pSmad and pNF-κB promoter reporter activity in 6 Gy IR MLE12 cells transfected with or without FPR2 siRNA. Data are expressed as the mean ± standard error (n = 3‒5, *p < 0.05, **p < 0.01, and ***p < 0.001).

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