Fig. 3: Autophagy was induced in endothelial cells of HLI mice. | Cell Death & Disease

Fig. 3: Autophagy was induced in endothelial cells of HLI mice.

From: Ischemia induces autophagy of endothelial cells and stimulates angiogenic effects in a hindlimb ischemia mouse model

Fig. 3

a Immunostaining of p62 was performed using quadriceps tissue from HLI mice. Nuclei were counterstained with hematoxylin. Scale bar = 25 μm. b–d, f Double immunofluorescence staining was performed using specific antibodies as indicated. p62 and CD31 (b); LC3 and CD31 (c); LC3 and dystrophin (d); and LC3 and LAMP2 (f). Nuclei were stained with Hoechst 33342. Scale bar = 20 μm. e, g The total LC3 signal and the overlapped LC3 signal with the indicated protein were quantified using Image J software and plotted. *p < 0.05, ***p < 0.001 versus control.

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