Fig. 3: CircMBOAT2 promoted the proliferation of CRC cells in vitro and in vivo. | Cell Death & Disease

Fig. 3: CircMBOAT2 promoted the proliferation of CRC cells in vitro and in vivo.

From: Circular noncoding RNA circMBOAT2 is a novel tumor marker and regulates proliferation/migration by sponging miR-519d-3p in colorectal cancer

Fig. 3

a Expression of circMBOAT2 in human colorectal normal epithelial cells (FHC) and four colorectal cancer cell lines (HCT-116, DLD-1, SW480, and HCT-8). b After transfection with si-circMBOAT2 RNA, the expression level of circMBOAT2 significantly reduced. c Expression levels of circMBOAT2 were detected in HCT-116 cells transfected with circMBOAT2 overexpression vector and empty vector (mock) by qRT-PCR. d CCK-8 assay showed that circMBOAT2 knockdown could inhibit the growth of HCT-8 and SW480 cells. e CCK-8 assay showed that the overexpression of circMBOAT2 could promote the growth of HCT-116 cells. f Colony formation assay showed that circMBOAT2 knockdown could inhibit the proliferation of HCT-8 and SW480 cells. g Colony formation assay showed that the overexpression of circMBOAT2 could promote the proliferation of HCT-116 cells. h Xenograft tumors were established by injecting SW480 cells stably expressing sh-circMBOAT2 compared with sh-NC (n = 4, each group). Representative images of nude mice are shown. i The sh-circMBOAT2 stably expressing group and the sh-NC group were used for xenograft tumorigenesis assay. The tumors of nude mice were harvested and evaluated after 4 weeks. j, k After subcutaneous injection of SW480 cells, tumor growth curves with the stable silencing of circMBOAT2 or the negative control are shown. The volumes (j) and tumor weights (k) were measured every 3 days after inoculation. **P < 0.01, ***P < 0.005, ****P < 0.001.

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