Fig. 5: CircMBOAT2 could serve as miRNA sponge. | Cell Death & Disease

Fig. 5: CircMBOAT2 could serve as miRNA sponge.

From: Circular noncoding RNA circMBOAT2 is a novel tumor marker and regulates proliferation/migration by sponging miR-519d-3p in colorectal cancer

Fig. 5

a, b qRT-PCR analysis of nuclear and cytoplasmic RNAs in HCT-8 (a) and SW480 (b) cells. c qRT-PCR analysis of circMBOAT2 and MBOAT2 RNA after treatment with RNase R enzyme in HCT-8 cells. d RNA immunoprecipitation (RIP) assay for the amount of circMBOAT2 in HCT-8 cells stably expressing GFP or Ago2. e RNA pull-down assay showed relative abundance of Ago2 after treated with biotinylated circMBOAT2 positive and negative probe in HCT-8 cells. f A schematic diagram showing the seven miRNAs which are most likely binding to circMBOAT2. g, h After the knockdown of circMBOAT2, the change in the expression levels of the target mRNAs were examined by qRT-PCR in HCT-8 (g) and SW480 (h) cells. i Schematic illustration shows the putative binding sites of miR-519d-3p with respect to circMBOAT2. j After co-transfection with circMBOAT2-WT or circMBOAT2-MUT and mimics, inhibitor, or NC, the relative luciferase activities were detected in 293T cells. k Expression of circMBOAT2 and miR-519d-3p in CRC tumor tissues had a negative correlation as detected by qRT-PCR. l Expression of circMBOAT2 and TROAP in CRC tumor tissues had a positive correlation as detected by qRT-PCR. **P < 0.01, ***P < 0.005, ****P < 0.001.

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