Fig. 2: XJB-5-131 administration attenuated kidney ischemic injury in mice.
From: XJB-5-131 inhibited ferroptosis in tubular epithelial cells after ischemia−reperfusion injury
a Scheme of the experiment. b Gross appearance on congestion area of representative kidneys from each group. c Analysis of the ratio of the left kidney to body weight. d XJB-5-131 decreased histologic injury in kidneys from mice with unilateral I/R at day 3. Representative image of periodic acid-Schiff (PAS) and tubular damage was scored semiquantitatively in PAS staining. Original magnification ×400. e The representative image of immunofluorescence staining of KIM-1 in kidney sections. Original magnification ×100. Representative image of Ki67 (f) and Sox9 (g) staining showed the proliferation in TECs after I/R injury at day 3. The number of Ki67- and Sox9-positive nuclei was measured in 6−8 visual fields. Original magnification ×400. N = 5/group. Scale bars = 50 μm. Data were presented as the means ± SEM. *p < 0.05, **p < 0.01,***p < 0.001.
