Fig. 7: Effect of 20(S)-Rh2E2 on AMPK-MAPKs signaling pathway. | Cell Death & Disease

Fig. 7: Effect of 20(S)-Rh2E2 on AMPK-MAPKs signaling pathway.

From: Novel ginsenoside derivative 20(S)-Rh2E2 suppresses tumor growth and metastasis in vivo and in vitro via intervention of cancer cell energy metabolism

Fig. 7

a 20(S)-Rh2E2 and 20(R)-Rh2E2 activated AMPK and MAPK signaling via activation phosphorylation of p38, p-JNK, and p-ERK. LLC-1 cells were treated with 20(S)/(R)-Rh2E2 (40 µM, 60 µM, 80 µM) for 24 h, respectively. Representative immunoblots and the protein quantification are shown; mean ± S.D., n = 3; *P < 0.05, **P < 0.01, ***P < 0.001, one-way ANOVA analysis. b 20(S)-Rh2E2 induced cell cytotoxicity through activation of AMPK and MAPK signaling. LLC-1 cells were preincubated with or without specific inhibitors for 2 h, then treated with 20(S)-Rh2E2 together with or without specific inhibitors. After 72 h, the cell viability was measured by MTT assay. c siRNA knockdown of AMPK and ERK in CMT167 mouse lung cancer cells. d 20(S)-Rh2E2-mediated cell cytotoxicity was dependent on AMPK and ERK expression. Cytotoxic effect of 20(S)-Rh2E2 in CMT167 cells transfected with control nonspecific siRNA or AMPK- or ERK-targeted siRNA. siRNA transfected cells were treated with DMSO (control) or 20(S)-Rh2E2 at indicated drug concentrations for 48 h and then subjected to MTT assay. Mean ± S.D. are from three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001).

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