Fig. 1: circ-GRB10 regulates NP cell functions through ERBB2/Erk signal pathway. | Cell Death & Disease

Fig. 1: circ-GRB10 regulates NP cell functions through ERBB2/Erk signal pathway.

From: The circular RNA circ-GRB10 participates in the molecular circuitry inhibiting human intervertebral disc degeneration

Fig. 1

a Collagen-II, aggrecan, MMP-13, ADAMT-5 expression were analyzed in circ-GRB10 or circ-GRB10 siRNA transfected cultured primary human NP cells using Immunofluorescence staining analysis. b The corresponding bar graphs show quantitative analysis of the relative fluorescent value of each group. n = 3 replicates per group, **p < 0.01, ***p < 0.001. Scale bar = 50 μm. c qRT-PCR showing the expression levels of collagen II, aggrecan, MMP13, ADAMT -5 in human NP cells after circ-GRB10 overexpression or knockdown. Three independent experiments are presented as mean ± SEM (error bars). **P < 0.01, ***P < 0.001. d The expression levels of Collagen-II, aggrecan, MMP13, ADAMT5, and p-Erk1/2 were detected by western blot; Quantitative analysis was shown in e, and three independent repeats were performed in each experiment. ***p < 0.001. f NP cell were co-transfected with circ-GRB10 and ERBB2 siRNA. Western blot assay showed that ERBB2 siRNA blocked the effect of circ-GRB10 on Collagen-II, aggrecan, MMP13 and ADAMT5 expression. Quantitative analysis was shown in g, and three independent repeats were performed in each experiment. ***p < 0.001. h NP cell were co-transfected with circ-GRB10 siRNA and ERBB2. Western blot assay showed that ERBB2 attenuated the effect of circ-GRB10 siRNA on Collagen-II, aggrecan, MMP13 and ADAMT5 expression. Quantitative analysis was shown in i, and three independent repeats were performed in each experiment. ***p < 0.001.

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