Fig. 5: ERBB2 regulate miR-141-3p expression in NP cells. | Cell Death & Disease

Fig. 5: ERBB2 regulate miR-141-3p expression in NP cells.

From: The circular RNA circ-GRB10 participates in the molecular circuitry inhibiting human intervertebral disc degeneration

Fig. 5

a miR-141-3p expression level in NP cells with ERBB2 overexpression or ERBB2 knockdown. Three independent experiments is presented as mean ± SEM (error bars). **P < 0.01. b NP cells overexpressing ERBB2 were treated with U0126 or not for one hour. Western blot was used to detect the phosphorylated level of Erk1/2. c, d NP cells overexpressing ERBB2 were treated with U0126 or not. qRT-PCR was used to detect the expression level of miR-141-3p and circ-GRB10. Three independent experiments are presented as mean ± SEM (error bars). **P < 0.01. e Schematic representation of mechanisms by which circ-GRB10 mediates IDD development. On the basis of findings described in the manuscript, miR-141-3p downregulates FUS level in NP cells, leading to circ-GRB10 decreased. This down-regulated circ-GRB10, in turn, enhanced miR-328-5p mediated suppression of ERBB2 in NP cells, leads to decreased Erk1/2 phosphorylation level. And the decreased Erk1/2 phosphorylation level enhances the generation of miR-141-3p in NP cells.

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