Fig. 6: cric-GRB10 attenuates IDD development in vivo. | Cell Death & Disease

Fig. 6: cric-GRB10 attenuates IDD development in vivo.

From: The circular RNA circ-GRB10 participates in the molecular circuitry inhibiting human intervertebral disc degeneration

Fig. 6

a Overview of the experimental set-up with injections of circ-GRB10, or their negative control at 1, and 7 days after surgery. b Six weeks after surgery, in vivo RNA FISH found circ-GRB10 located in the NP region. Blue fluorescence (4,6-diamidino-2-phenylindole, DAPI) indicating cell nucleus; Red fluorescence indicating circ-GRB10. Scale bar = 200 μm. c Changes in disc height index (DHI) of the indicated groups after needle puncture. The DHI was measured at 0, 1, 6, and 12 weeks. A significant decrease of the DHI% was observed in all puncture groups at 1 week after surgery (P < 0.01). A significant increase in DHI% was noted at 6 and 12 weeks post surgery in rat treated with circ-GBR10 (P < 0.001). d CT scan of the indicated groups were obtained 12 weeks after needle puncture. e The intervertebral disc degeneration evaluated by Safranin O staining. Scale bar = 500 µm. f qRT-PCR showed that the increased levels of FUS, ERBB2 and the decreased level of miR-141-3p in the punctured discs treated with circ-GRB10. Three independent experiments are presented as mean ± SEM (error bars). ***P < 0.001. g Western blot showing the expression levels of collagen II, aggrecan, p-Erk1/2, MMP13, ADAMT-5 in rat NP tissues. h Immunostaining for collagen-II and aggrecan in IDD model treated by circ-GRB10 at 6 and 12 weeks. Scale bar = 100 µm.

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