Fig. 4: TTN and lncRNA-TTN-AS1 promoted SKCM tumor growth and metastasis in vivo. | Cell Death & Disease

Fig. 4: TTN and lncRNA-TTN-AS1 promoted SKCM tumor growth and metastasis in vivo.

From: Long noncoding RNA TTN-AS1 facilitates tumorigenesis and metastasis by maintaining TTN expression in skin cutaneous melanoma

Fig. 4

a Xenograft assays of B16F10 cells treated with shTTN, shTTN-AS1 or vector were performed on C57BL/6 mice (n = 6), representative tumors, tumor growth curves, and the weight of tumors were shown. b, c Immunohistochemical staining was performed to detect the expression of Ki-67 (b) and TTN proteins (c) in tumor tissues. Scale bar, 40 μm and 100 μm. d The lung and kidney metastatic sites were determined by GFP-based fluorescence imaging. Scale bar, 5 mm. e Inflammatory infiltration of lungs and kidneys were detected by hematoxylin and eosin staining. Scale bar, 100 μm. Experiments were performed three times and data were presented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001. Student t test.

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