Fig. 5: LncRNA-TTN-AS1 modulated the properties of SKCM cells by promoting TTN expression.

a, b Colony formation (a) and CCK-8 (b) assays were used to test the proliferation and viability of B16F10 cells transfected with lncRNA-TTN-AS1, lncRNA-TTN-AS1 + shTTN or vector. Scale bar, 400 μm. c, d Flow cytometry was used to detect cell cycle (c) and apoptosis (d) of B16F10 cells transfected with lncRNA-TTN-AS1, lncRNA-TTN-AS1 + shTTN or vector. e, f Transwell migration/invasion (e) and wound healing (f) assays showed the migration and invasion of B16F10 cells transfected with lncRNA-TTN-AS1, lncRNA-TTN-AS1 + shTTN or vector. Scale bar, 200 μm. Experiments were performed three times and data were presented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001. Student t test.