Fig. 6: LncRNA-TTN-AS1 regulated TTN expression by activating TTN promoter activity and stabilizing TTN mRNA. | Cell Death & Disease

Fig. 6: LncRNA-TTN-AS1 regulated TTN expression by activating TTN promoter activity and stabilizing TTN mRNA.

From: Long noncoding RNA TTN-AS1 facilitates tumorigenesis and metastasis by maintaining TTN expression in skin cutaneous melanoma

Fig. 6

a Methylation status (left panel) and relative expression (right panel) of lncRNA-TTN-AS1 in different cell lines. b Upper panel: a schematic map of TTN promoter region and luciferase reporter. Bottom panel: dual luciferase report assay was used to detect TTN promoter activity in 293T cells. c The half-life of TTN mRNA was measured by qRT-PCR after blocking new RNA synthesis with α-amanitin and normalized to 18s rRNA. d, e Subcellular distribution of TTN protein and mRNA in B16F10 cells treated with overexpressed TTN-AS1 plasmid or vector were analyzed by immunofluorescence (d) and nuclear/cytoplasmic fractionation (e) assays. Scale bar, 10 μm. Experiments were performed three times and data were presented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001. Student t test. f Schematic representation of the mechanism by which lncRNA-TTN-AS1 interacts with TTN.

Back to article page