Fig. 4: DNER activates the Wnt/β-catenin signalling pathway and is positively correlated with β-catenin. | Cell Death & Disease

Fig. 4: DNER activates the Wnt/β-catenin signalling pathway and is positively correlated with β-catenin.

From: DNER promotes epithelial–mesenchymal transition and prevents chemosensitivity through the Wnt/β-catenin pathway in breast cancer

Fig. 4

a, b Western blotting detected the expression of Notch1, p-GSK3β, GSK3β and β-catenin after DNER knockdown or DNER-overexpressing in BC cells. c. Total proteins (β-catenin and Snail) nuclear proteins (β-catenin and Snail) in DNER knockdown cells were assayed with western blotting. d The mRNA levels of Survivin, c-Myc and LEF1 were detected by qRT-PCR. e The staining of DNER and β-catenin in BC tissue by IHC (magnification ×200). f Correlation analyses of protein expression levels between DNER and β-catenin. g Kaplan–Meier survival analysis of BC patients was performed with DNERHighβ-cateninHigh and DNERLowβ-cateninLow expression. The values are the mean ± SD from three independent experiments. **p < 0.01, ***p < 0.001 vs the corresponding group.

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