Fig. 2: ABT-199/BZB-induced cell death is apoptotic. | Cell Death & Disease

Fig. 2: ABT-199/BZB-induced cell death is apoptotic.

From: The BCL-2 selective inhibitor ABT-199 sensitizes soft tissue sarcomas to proteasome inhibition by a concerted mechanism requiring BAX and NOXA

Fig. 2

a, b RD and SW982 cells were incubated in the presence of 5 nM BZB and 15 µM ABT-199 alone or in combination. After 48 h the percentage of cells with hypodiploid DNA (subG1) was assessed by flow cytometry. c, d RD and SW982 cells were incubated with 5 nM BZB and/or 15 µM ABT-199 for 24 h. Subsequently, exposure of phosphatidylserine (Annexin V) and loss of mitochondrial membrane potential (TMRM) were analyzed by flow cytometry. e SW982 cells were incubated without or with ABT-199/BZB for 24 h. Immunofluorescence microscopy shows activation/clustering of the effectors BAX, BAK, and BOK (green) in response to ABT-199/BZB.

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