Fig. 5: Both NOXA and BAX mediate synergistic apoptosis induction by ABT-199/BZB. | Cell Death & Disease

Fig. 5: Both NOXA and BAX mediate synergistic apoptosis induction by ABT-199/BZB.

From: The BCL-2 selective inhibitor ABT-199 sensitizes soft tissue sarcomas to proteasome inhibition by a concerted mechanism requiring BAX and NOXA

Fig. 5

SW982/wt and SW982/BAXKO or SW982/NOXAKO were transfected with siRNA-NOXA or siRNA-BAX and incubated with 5 nM BZB and/or 15 µM ABT-199. Apoptosis induction was evaluated after 24 h by measuring a phosphatidylserine exposure (Annexin V-APC+) and c loss of mitochondrial membrane potential (TMRMlow). b, d Relative reduction of phosphatidylserine exposure (b) and loss of mitochondrial membrane potential (d) by siRNA-mediated knock-down of NOXA and BAX in wt and BAK-, BOK-, or BAX-deficient SW982 cells in response to treatment with ABT-199/BZB.

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