Fig. 8: Activation of ER-stress and reduction of protein synthesis in 3Tg-iAstro vs WT-iAstro cells. | Cell Death & Disease

Fig. 8: Activation of ER-stress and reduction of protein synthesis in 3Tg-iAstro vs WT-iAstro cells.

From: Proteomic analysis links alterations of bioenergetics, mitochondria-ER interactions and proteostasis in hippocampal astrocytes from 3xTg-AD mice

Fig. 8

a Real-time PCR of ER-stress/UPR response-related genes Atf4 (p = 0.037), Atf6 (p = 0.02), Herp (p = 0.031) and Xbp1 (p = 0.059) of 3Tg-iAstro vs WT-iAstro cells. Data are expressed as mean ± SEM ΔC(t), n = 14 for WT-iAstro, n = 12 biological replicates for 3Tg-iAstro cells. b WT and 3Tg-iAstro were pulsed with puromycin for 3 h. Where indicated, cells were also treated with cycloheximide (CHX, 10 μM) ten minutes before adding puromycin. Anti-puromycin antibody was used to detect neo-synthesized peptides. Ponceau staining was used for band normalization. c Quantification of anti-puromycin detected bands. Data are expressed as mean ± SEM, six and four independent cultures were used for WT and Tg-iAstro, respectively (p = 0.033; two-tailed unpaired Students’s t test with Mann–Whitney correction).

Back to article page