Fig. 6: Effects of ELA on Aldosterone-induced NADPH oxidase/ROS/NLRP3 pathway in HK2 cells. | Cell Death & Disease

Fig. 6: Effects of ELA on Aldosterone-induced NADPH oxidase/ROS/NLRP3 pathway in HK2 cells.

From: ELABELA attenuates deoxycorticosterone acetate/salt-induced hypertension and renal injury by inhibition of NADPH oxidase/ROS/NLRP3 inflammasome pathway

Fig. 6

The HK2 cells were pretreated with ELA peptide (1 nM) for 1 h and then treated by Aldosterone (0.1 µM) for 24 h. a, b representative confocal fluorescent images of co-localization (yellow) between Nlrp3 (green)/Caspase-1 (red), Nlrp3 (green)/ASC (red) and summarized co-localization coefficient (n = 5 per group). Scale bar, 10 µm. c Representative immunoblots of cleaved Caspase-1 and summarized intensities of blots (n = 5 per group). d ROS production (n = 6 per group). e Intracellular NADP+/NADPH (n = 6 per group). *P < 0.05 versus Ctrl group; #P < 0.05 versus DOCA/salt group. ROS reactive oxygen species, NADPH nicotinamide adenine dinucleotide phosphate.

Back to article page