Fig. 2: Anlotinib induces G2/M phase arrest and apoptosis. | Cell Death & Disease

Fig. 2: Anlotinib induces G2/M phase arrest and apoptosis.

From: Reactive oxygen species mediate anlotinib-induced apoptosis via activation of endoplasmic reticulum stress in pancreatic cancer

Fig. 2

a–d Anlotinib-induced G2/M phase arrest. The cells were treated with anlotinib for 24 h and analysed by flow cytometry after staining with PI. e–h After treatment with anlotinib, the cells were stained with PI and Annexin-V-FITC to detect apoptosis, followed by flow cytometry. The population of cells in the lower left quadrant shows the live cells (Annexin V-/PI-), population in lower right quadrant shows the early apoptotic cells and that in upper right quadrant shows the late apoptotic or dead cells. Cells in state of early apoptosis, late apoptosis and death cells were counted in apoptotic cells. i Western blotting experiments detected several markers that reflect the apoptosis level of cells. The data are presented as the mean ± SD from 3 independent experiments (*p < 0.05; **p < 0.01; ***p < 0.001).

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