Fig. 5: Sp results in recovery of motion and phototactic defects in Parkinsonian flies.

a Fifteen days old park¹³ and wild type flies were subjected to negative geotaxis movement and the distance traveled through a vertical chamber was graphically represented as mean ± s.e.m.; n = 5, **P (t-test) <0.001. b The relative distribution or no net movement (Neutral) of wild type and mutant flies across the illuminated (Light) and darkened (Dark) arms of a Y-maze was observed and plotted as mean ± s.e.m.; n = 5, **P (t-test) < 0.001. c The fold change in mt-roGFP fluorescence intensities (FI) of the dopaminergic neurons in wild type and park¹³ flies exposed to 1 mM L-DOPA alone or in combination with 2.5 mM Sp, compared to untreated wild type control. Bars represents mean ± s.e.m.; n = 10, **P (t-test) < 0.001, ***P (t-test) <0.0001. d, e Temporal determination of negative geotactic movement of wild type (+/+) (d) or mutant (e) flies continuously fed with 1 mM L-DOPA, 2.5 mM Sp or combination. Each data point represent mean ± s.e.m.; n = 5, P < 0.001.