Fig. 6: Impact of A-485 on the expressions of gluconeogenesis-related transcriptional factors. | Cell Death & Disease

Fig. 6: Impact of A-485 on the expressions of gluconeogenesis-related transcriptional factors.

From: Selective inhibition of CBP/p300 HAT by A-485 results in suppression of lipogenesis and hepatic gluconeogenesis

Fig. 6

a Primary mouse hepatocytes were incubated with 3 μM A-485 and 100 μM 8-Br-cAMP for 1 h. The phosphorylation levels of CREB, AMPK, and TORC2 were detected by western blot. b The interaction of CREB with CBP and TORC2 was detected in HepG2 cells treated with 3 μM A-485 by coimmunoprecipitation (CoIP). c CRE reporter activity was detected in HepG2 cells treated with 3 μM A-485. d–g mRNA expressions of gluconeogenesis-related transcriptional factors or coactivators in primary mouse hepatocytes treated with 3 μM A-485 and 100 μM 8-Br-cAMP for 8 h. h Protein expressions of FOXO1, C/EBPα, and PGC1α in primary mouse hepatocytes treated with 3 μM A-485 and 100 μM 8-Br-cAMP for 12 h. i FOXO1 and C/EBPα protein expressions in the liver isolated from vehicle- and A-485-administered mice. j ChIP-qPCR analysis of the occupancy of the G6Pc promoter with FOXO1 in the liver isolated from vehicle- and A-485-administered mice. Data are expressed as means ± SEM for three independent experiments. *P < 0.05, ***P < 0.001 vs. corresponding control group.

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