Fig. 5: MiR-378-3p directly targeted the 3′UTR of PDK1 and Caspase9. | Cell Death & Disease

Fig. 5: MiR-378-3p directly targeted the 3′UTR of PDK1 and Caspase9.

From: miR-378-3p maintains the size of mouse primordial follicle pool by regulating cell autophagy and apoptosis

Fig. 5

a The binding position of miR-378 in target genes (PDK1 and Caspase9) and corresponding mutated sequences; b, c RLU (relative light unit) detected using the Dual Luciferase Assay System; d The protein expression of target PDK1 from 16.5 dpc to 3 dpp; e, f The protein expression of target PDK1 and downstream mTOR after 1.5 days’ starvation and 2 days’ starvation by western blotting; g, h The protein expression of target CASP9 and downstream CASP3 after 1.5 days’ starvation and 2 days’ starvation by western blotting.

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