Fig. 7: MiR-378-3p knockdown inhibited autophagy and induced apoptosis. | Cell Death & Disease

Fig. 7: MiR-378-3p knockdown inhibited autophagy and induced apoptosis.

From: miR-378-3p maintains the size of mouse primordial follicle pool by regulating cell autophagy and apoptosis

Fig. 7

a Immunofluorescence staining of in vitro cultured mouse ovaries after miR-378-3p knockdown. Germ cells were stained red with anti-Mvh antibody, nuclei were stained blue with Hochest 33342; b The total number of germ cells after miR-378-3p knockdown; c The percentage of germ cells in Cyst and primordial follicles after miR-378-3p knockdown; d The expression of miR-378-3p after miR-378-3p knockdown by miRNA qRT-PCR; eh Protein expression of autophagy-related genes (LC3B, PDK1,mTOR and p-mTOR) by western blotting; i Autophagic flux after Bafilomycin A1 (BA1) treatment; jl Protein expression of apoptosis-related genes (BAX, BCL-2, CASP9, and CASP3) by western blotting.

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