Fig. 1: Madecassic acid decreases the number of γδT17 cells in the colons of mice with dextran sulfate sodium (DSS)-induced colitis.

Colitis was induced in female C57BL/6 mice by drinking with 2.5% DSS for 7 days, and followed by normal drinking water for 3 days. Madecassic acid (MA, 25 mg/kg) and cyclosporin A (CsA, 25 mg/kg) were orally gavaged for 10 days consecutively. a Disease activity index (DAI). b Colon length. c Myeloperoxidase (MPO) activity. d The expression of Il17a mRNA as assessed by real-time PCR. e The levels of IL-17A as assessed by enzyme-linked immunosorbent assay. f, g The representative flow cytometry and percentage of CD3⁺IL-17⁺ cells in the mononuclear cells of lamina propria from mouse colon tissues. h The representative flow cytometry of IL-17 expression in CD8⁺ T cells (Tc17), CD4⁺ T cells (Th17), and γδTCR⁺ T cells (γδT17) in CD3⁺ cells. i The percentages of IL-17A⁺ subpopulations present in the CD8⁺, CD4⁺, and γδTCR⁺ T cells. j The absolute number of Tc17, Th17, and γδT17 cells in CD3 + cells (10⁴). The data are expressed as means ± SEM of six mice per group. ^##P < 0.01 versus the normal group; ^*P < 0.05, ^**P < 0.01 versus the DSS group, ^$$P < 0.01 versus the Tc17 cell group.