Fig. 3: Madecassic acid inhibits the activation of γδT17 cells in vitro. | Cell Death & Disease

Fig. 3: Madecassic acid inhibits the activation of γδT17 cells in vitro.

From: Inhibition of the activation of γδT17 cells through PPARγ–PTEN/Akt/GSK3β/NFAT pathway contributes to the anti-colitis effect of madecassic acid

Fig. 3

The purified γδT cells were cultured with IL-1β (10 ng/mL) and IL-23 (10 ng/mL) for 72 h in the presence of madecassic acid (MA, 1, 3, or 10 μM). a The representative flow cytometry and percentages of IL-17A+ subpopulations present in the γδTCR+ T cells. b The expression levels of the cytokines in γδT17 cells, including Il17a, Il17f, Il21, and Il22, as assessed by real-time PCR. c The expression levels of the transcription factors of γδT17 cells, including Rorc, Sox4, Sox13, and Plzf, as assessed by real-time PCR. d The expression levels of the surface receptors of γδT17 cells, including Il1r1, Il23r, Ccr2, and Ccr6, as assessed by real-time PCR. The data are expressed as means ± SEM from three independent experiments. *P < 0.05, **P < 0.01 versus the untreated group; #P < 0.05, ##P < 0.01 versus IL-1β and the IL-23-stimulated group.

Back to article page