Fig. 4: Screening of the signaling pathways accounting for madecassic acid inhibiting the activation of γδT17 cells. | Cell Death & Disease

Fig. 4: Screening of the signaling pathways accounting for madecassic acid inhibiting the activation of γδT17 cells.

From: Inhibition of the activation of γδT17 cells through PPARγ–PTEN/Akt/GSK3β/NFAT pathway contributes to the anti-colitis effect of madecassic acid

Fig. 4

The γδT cells were stimulated with IL-1β (10 ng/mL) and IL-23 (10 ng/mL) for 72 h in the presence or absence of madecassic acid (MA, 10 μM). a The fluorescent images of the relative protein phosphorylation and expression as detected by the phospho explorer antibody array. Data are presented as fold change after normalization to untreated cells. b, g The expression and phosphorylation levels of PPARγ, PTEN, PI3K, AKT, GSK3β, and mTOR, as detected by western blot. h The localization of NFATc1 as visualized by immunofluorescence analysis (scale bar, 20 μm). i Schematic diagram of activated or deactivated proteins of the signaling pathway upon MA treatment (according to www.phosphosite.org). The data are expressed as means ± SEM from six technical replicates in microarray experiments or from three independent experiments. **P < 0.01 versus the untreated group; ##P < 0.01 versus IL-1β and IL-23-stimulated group.

Back to article page