Fig. 6: Madecassic acid inhibits the PI3K–Akt/NFAT signal pathway in γδT17 cells by activating PPARγ. | Cell Death & Disease

Fig. 6: Madecassic acid inhibits the PI3K–Akt/NFAT signal pathway in γδT17 cells by activating PPARγ.

From: Inhibition of the activation of γδT17 cells through PPARγ–PTEN/Akt/GSK3β/NFAT pathway contributes to the anti-colitis effect of madecassic acid

Fig. 6

The γδT cells were stimulated with IL-1β (10 ng/mL) and IL-23 (10 ng/mL) for 72 h in the presence or absence of madecassic acid (MA, 10 μM) or in combination with GW9662 (1 μM) or rosiglitazone (RGZ, 2 μM). a The representative flow cytometry and percentages of IL-17A+ subpopulations present in the γδTCR+ T cells. b, c The expression levels of Il17a and Rorc as assessed by real-time PCR. d–g The expression and phosphorylation levels of PPARγ, PTEN, PI3K, AKT, GSK3β, and mTOR, as detected by western blot. f The expression level of Pten as assessed by real-time PCR. h The localization of NFATc1, as visualized by immunofluorescence analysis (scale bar, 20 μm). The data are expressed as means ± SEM from three independent experiments. *P < 0.05, **P < 0.01 versus the indicated group.

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