Fig. 1: CCL18 promotes OSCC progression by regulating LINC00319.

a Scatter plot of differentially expressed lncRNAs and mRNAs in CCL18-stimulated HSC6 cells, which was determined by lncRNA sequencing. b Heatmaps of differentially expressed lncRNAs. c qRT-PCR detected four upregulated lncRNAs (i.e., LINC00649, LINC00319, RP13-516M14.1, and RP11-454K7.1) in rCCL18-stimulated HSC6 cells. d qRT-PCR detected the expression level of LINC00319 in rCCL18-stimulated CAL27 cells. e qRT-PCR detected the expression level of LINC00319 in OSCC cells and HOK cell (n = 3), and TCGA database analyzed the expression of LINC00319 in HNSCC tissues. f The effective siLINC00319 fragments were screened in HSC6 cells and CAL27 cells by qRT-PCR (n = 3). g CCK8 assay detected the proliferation in rCCL18-stimulated OSCC cells with or without siLINC00319 silencing (n = 3). h, i Transwell assay evaluated the migration (h) and invasion (i) of cells described in g (n = 5). j Angiogenesis assay described the angiogenesis of HUVEC cells (i.e., number of branches, meshes, junctions, and total meshes area) which co-cultured with the supernatant of OSCC cells described in g (n = 3). k The protein level of EMT and angiogenesis markers in cells described in g was detected using western blotting (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.