Fig. 2: LINC00319 promotes the progression of OSCC through sponging miR-199a-5p. | Cell Death & Disease

Fig. 2: LINC00319 promotes the progression of OSCC through sponging miR-199a-5p.

From: CCL18-induced LINC00319 promotes proliferation and metastasis in oral squamous cell carcinoma via the miR-199a-5p/FZD4 axis

Fig. 2

a The mRNA level of miR-199a-5p and let-7a-5p in OSCC cells with or without transfection of siLINC00319 (n = 3). b Representative FISH image of the location of LINC00319 (Red), miR-199a-5p (green), and nuclei (blue) in HSC6 cells. c The putative site 1 of miR-199a-5p in the LINC00319 3’UTR was analyzed by dual-luciferase reporter assays (n = 3). d The mRNA level of LINC00319 in OSCC cells infected with LINC00319-silencing lentivirus (n = 3). e The mRNA level of miR-199a-5p in OSCC cells infected with miR-199a-5p inhibitor (n = 3). f CCK8 assay detected that the proliferation of LINC00319-silenced cells with or without miR-199a-5p inhibitor, as well as the negative control group (n = 3). g, h Cells were treated as described in f. The migration cells number (g) and invasion cells number (h) in transwell assay were analyzed (n = 5). i The number of branches, meshes, junctions, and total meshes area in HUVEC cells co-cultured with the supernatant of OSCC cells described in f (n = 3). j The protein expression of EMT markers and angiogenesis markers in cells described in f was detected using western blotting (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.

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