Fig. 3: Upregulated FZD4 mediated by miR-199a-5p and LINC00319 promotes the progression of OSCC and reverses the inhibitory effects of LINC00319 silencing in OSCC cells.

a Venn diagram identified 7 genes (i.e., HOXA7, ETS1, FZD4, FZD6, CTNNA2, LAMC1, and DUSP14) to be the overlap between lncRNA-seq results and miR-199a-5p targets. The qRT-PCR analyzed the mRNA expression of these 7 genes in HSC6 cells transfected with siLINC00319 and miR-199a-5p mimic, as well as that in corresponding control cells (n = 3). b Western blotting detected the protein expression of FZD4 in HSC6 and CAL27 cells with treated as a (n = 3). c The putative site of miR-199a-5p in the FZD4 3’UTR was analyzed by dual-luciferase reporter assays (n = 3). d CCK8 assay detected the proliferation of HSC6 and CAL27 cells treated in different group (LV-NC + siNC, LV-FZD4 + siNC, LV-NC + siLINC00319, and LV-FZD4 + siLINC00319; n = 3). e, f Cells were treated as described in d. The migration cells number (e) and invasion cells number (f) in transwell assay were analyzed (n = 5). g The number of branches, meshes, junctions, and total meshes area in HUVEC cells co-cultured with the supernatant of OSCC cells described in d (n = 3). h Western blotting evaluated the protein expression of EMT markers and angiogenesis markers in cells described in d (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.