Fig. 1: Nuclear factor E2-related factor 2 (Nrf2) cascade activation by 4-OI in osteoblasts. | Cell Death & Disease

Fig. 1: Nuclear factor E2-related factor 2 (Nrf2) cascade activation by 4-OI in osteoblasts.

From: Four-octyl itaconate activates Nrf2 cascade to protect osteoblasts from hydrogen peroxide-induced oxidative injury

Fig. 1

Human osteoblastic OB-6 cells (a–g) or the primary murine osteoblasts (h, i) were treated with 4-OI (10/25 μM) or vehicle control (0.2% of DMSO, “Veh”) and cultured for applied time periods; Keap1–Nrf2 association was tested by co-immunoprecipitation (Co-IP assay) (a). Expression of listed proteins in cytosol fraction lysates and nuclei fraction lysates was tested by western blotting (b, d, g, h), with the expression of listed mRNAs tested by qPCR (c, f, i). The relative ARE reporter luciferase activity was tested as well (e). The morphology of murine osteoblasts with ALP staining is shown (h, the left panel). Expression of the listed proteins was quantified and normalized to the loading control (a, b, d, g, h). Quantified values were mean ± standard deviation (SD, n = 5). “n.s.” stands for no statistical difference (c). *P < 0.05 vs. “Veh” treatment. Experiments were repeated three times, with similar results obtained. Bar = 100 μm (h).

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