Fig. 2: H2O2-induced oxidative injury was inhibited by 4-OI in osteoblasts. | Cell Death & Disease

Fig. 2: H2O2-induced oxidative injury was inhibited by 4-OI in osteoblasts.

From: Four-octyl itaconate activates Nrf2 cascade to protect osteoblasts from hydrogen peroxide-induced oxidative injury

Fig. 2

Human osteoblastic OB-6 cells (a–c) or primary murine osteoblasts (d–f) were pretreated for 2 h with 4-OI (10/25 μM) or vehicle control (“Veh”), followed by H2O2 (400 μM) stimulation. Cells were further cultured for applied time periods, reactive oxygen species (ROS) production (tested by CellROX intensity, a, d), the GSH/GSSG ratio (b, e), and single-strand DNA (ssDNA) contents (ELISA OD, c, f) were tested. Quantified values were mean ± standard deviation (SD, n = 5). “C” stands for the untreated control cells. *P < 0.05 vs. “C” cells. #P < 0.05 vs. cells with H2O2 stimulation but “Veh” pretreatment. Experiments were repeated three times, with similar results obtained. Bar = 100 μm (a, d).

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