Fig. 3: AS serum contained elevated AOPPs levels and could stimulate ROS production in MSCs. | Cell Death & Disease

Fig. 3: AS serum contained elevated AOPPs levels and could stimulate ROS production in MSCs.

From: Oxidative stress-mediated mitochondrial dysfunction facilitates mesenchymal stem cell senescence in ankylosing spondylitis

Fig. 3

a The levels of AOPPs were higher in AS serum (108.0 ± 11.69 µmol/L) than in HD serum (44.63 ± 9.41 µmol/L); p < 0.001, n = 20. b–e The levels of AOPPs in AS serum were positively correlated with the level of CRP (r = 0.6055, p < 0.01) (b), BASDAI scores (r = 0.5635, p < 0.01) (c), the percentage of SA-β-gal-positive cells after treatment with AS serum (r = 0.7148, p < 0.01) (d), and the expression of the protein p53 in MSCs treated with AS serum (r = 0.65458, p < 0.01) (e–g). Flow cytometry detection revealed that the total ROS level was enhanced in MSCs treated with AS serum compared with those treated with HD serum. h AS serum-treated MSCs displayed a stronger signal for ROS than HD serum-treated MSCs under a fluorescence microscope (scale bar = 20 µm). The results in a, f, and g are presented as the means ± SD (n = 20, determined by independent-sample t tests). The data in b–e were determined by Pearson correlation and linear regression analysis (n = 20). All experiments were performed three independent times, *p < 0.05, **p < 0.01.

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