Fig. 4: ROS inhibition via NAC rescued MSCs from the senescence caused by AS serum. | Cell Death & Disease

Fig. 4: ROS inhibition via NAC rescued MSCs from the senescence caused by AS serum.

From: Oxidative stress-mediated mitochondrial dysfunction facilitates mesenchymal stem cell senescence in ankylosing spondylitis

Fig. 4

a, b NAC reduced the ROS level in AS serum-treated MSCs to the level in HD serum-treated MSCs. c, d NAC rescued the extent of SA-β-gal staining of MSCs (black arrow) induced by AS serum (scale bar = 50 µm). e, f NAC decreased the expression levels of the cell cycle arrest-related proteins p53, p21, and p16 in AS serum-treated MSCs to the levels in HD serum-treated MSCs. The results are presented as the means ± SD (n = 10, determined by independent-sample t tests). All experiments were performed three independent times, *p < 0.05, **p < 0.01.

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