Fig. 6: Mitoquinone decreased the ROS level and reversed the senescence of MSCs caused by AS serum. | Cell Death & Disease

Fig. 6: Mitoquinone decreased the ROS level and reversed the senescence of MSCs caused by AS serum.

From: Oxidative stress-mediated mitochondrial dysfunction facilitates mesenchymal stem cell senescence in ankylosing spondylitis

Fig. 6

a Mitochondrial ROS evaluation with MitoSOX showed that AS serum increased the mitochondrial ROS levels of MSCs and that MQ pretreatment eliminated this effect (scale bar = 40 µm). b, c The total ROS level detected by the probe DCFH-DA showed that MQ reduced the total ROS level of AS serum-treated MSCs to the level of HD serum-treated MSCs. d, e The SA-β-gal staining assay indicated that MQ decreased the percentage of SA-β-gal-positive MSCs (black arrow) treated with AS serum (scale bar = 50 µm). f, g Western blot analysis revealed that MQ reversed the expression of the cell cycle arrest-related proteins p53, p21, and p16 in MSCs treated with AS serum. The results are presented as the means ± SD (n = 10, determined by independent-sample t tests). All experiments were performed three independent times, *p < 0.05, **p < 0.01.

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