Fig. 1: Characterization and validation of BAX/BAK DKO hiPSCs cell death phenotypes.
From: Modeling the function of BAX and BAK in early human brain development using iPSC-derived systems
a Immunoblot shows complete KO of BAX and BAK in DKO clones and full expression of proteins control clones normalized to beta-actin. b Pluritest assay shows two control and two DKO clones as being pluripotent compared to non-iPSC control. c Apoptosis/necrosis assay shows that DKOs have significant decrease in apoptosis after three independent experiments. Error bars: SEM. Each symbol represents percentage of cells in the field of view that were positive for apoptosis or necrosis. d hiPSCs DKO clones demonstrate increased cell survival with 24-h 20 µM DNA-damaging etoposide compared to control clones as shown by two-way ANNOVA. e hiPSCs DKO clones have increased cell survival compared to control when exposed for 24 h with 160 µM CCCP. Four independent experiments were conducted for etoposide and CCCP exposures. Each symbol represents an individual experiment and the percent survival for that clone. Error bars: SEM.
