Fig. 7: RFXAP silencing attenuated the DNA damage induced by fisetin in pancreatic cancer. | Cell Death & Disease

Fig. 7: RFXAP silencing attenuated the DNA damage induced by fisetin in pancreatic cancer.

From: Fisetin inhibits proliferation of pancreatic adenocarcinoma by inducing DNA damage via RFXAP/KDM4A-dependent histone H3K36 demethylation

Fig. 7

a mRNA levels of RFXAP in control and RFXAP silencing (si1, si2, si3) PANC-1 were detected by qRT-PCR. The results are presented as the mean ± SD (n = 3), **P < 0.01. ***P < 0.001. b RFXAP protein expressions in RFXAP silencing PANC-1 cells were detected by western blot analysis. c Expression levels of KDM4A and H3K36me3 in RFXAP silencing PANC-1 cells, with or without fisetin treatment, were detected by western blot analysis. d Analyses of DNA damage by observation of the immunofluorescence of γ-H2AX (green) in PDAC cells. RFXAPi-PANC-1 cells were treated with fisetin (100 μM) for 48 h. The nucleus were counterstained with DAPI (blue). Scale bars 10 μm. e Comparative changes in the γ-H2AX spots number in control and RFXAPi-PANC-1 cells with treatment groups. The spots were counted by manual scoring. Results are mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001, #ns no significance. f CCK-8 asssy of RFXAP silencing PANC-1 cells. Cells were treated with fisetin (0 or 100 μM) for 48 h, respectively. The absorbance was measured at 450 nm. Data are presented as mean ± SD (n = 3); *P < 0.05.

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