Fig. 4: Elevation of Gli4 reverses the LINC01106 knockdown-induced progression retard in CRC. | Cell Death & Disease

Fig. 4: Elevation of Gli4 reverses the LINC01106 knockdown-induced progression retard in CRC.

From: LINC01106 drives colorectal cancer growth and stemness through a positive feedback loop to regulate the Gli family factors

Fig. 4

a and b As it turned out in colony formation and EdU assays, LINC01106 silencing-caused proliferation suppression could be reversed by overexpressing Gli4. c In Transwell migration assay, suppressed CRC cell migration resulted from LINC01106 blockade was promoted via pcDNA3.1/Gli4. d Western blot assay was used to examine migrating-related proteins and EMT markers in cells transfected with sh-LINC01106 or co-transfected with sh-LINC01106 and pcDNA3.1/Gli4. e and f Analyses by qRT-PCR and western blot indicated depressed level of stemness-related factors in LINC01106-knockdown cells was restored via pcDNA3.1/Gli4. g Sphere formation assay showed that pcDNA3.1/Gli4 attenuated the effects of LINC01106 knockdown on CRC cell sphere formation. Data shown as mean ± SD were collected from three independent experiments. **P < 0.01 indicated data had statistical significance.

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