Fig. 5: Nuclear LINC01106 recruits FUS to transcriptionally activate Gli1 and Gli2. | Cell Death & Disease

Fig. 5: Nuclear LINC01106 recruits FUS to transcriptionally activate Gli1 and Gli2.

From: LINC01106 drives colorectal cancer growth and stemness through a positive feedback loop to regulate the Gli family factors

Fig. 5

a Western blot assay identified that LINC01106 suppression affected the level of Gli1 and Gli2 protein, but not PTCH1 and Shh. b The effect of LINC01106 on the mRNA level of Hedgehog pathway factors was detected by qRT-PCR. c RNA pull-down followed by silver staining exhibited the LINC01106-interacting proteins. d and e RNA pull-down followed by western blot assay and RIP assay together supported that LINC01106 combined with FUS. f Using JASPAR online tools, the DNA motif of FUS was presented. g The FUS-binding sites in Gli1 and Gli2 promoters were displayed. h and i The specific functional binding region in Gli1 and Gli2 promoters was confirmed by conducting luciferase reporter assays. j ChIP assay described the occupancy of FUS in the promoter region of Gli1 and Gli2. Data shown as mean ± SD were collected from three independent experiments. **P < 0.01 indicated data had statistical significance. n.s. indicated difference had no statistical significance.

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