Fig. 2: p65 facilitates the transcriptional activation of Shh in LPS-treated MLE-12 cells. | Cell Death & Disease

Fig. 2: p65 facilitates the transcriptional activation of Shh in LPS-treated MLE-12 cells.

From: Mesenchymal stem cells reverse EMT process through blocking the activation of NF-κB and Hedgehog pathways in LPS-induced acute lung injury

Fig. 2

a RT-qPCR detected the level of Shh in LPS-treated MLE-12 cells with p65 overexpression or inhibition. b ChIP assay examined the enrichment of Shh promoter induced by p65 or IgG in LPS-treated MLE-12 cell. c, d JASPAR predicted the DNA motif of p65 and its binding sites in Shh promoter. e ChIP assay detected the enrichment of Shh promoter on P1 or P2 in p65- or IgG-precipitated compounds in LPS-treated MLE-12 cells. f Luciferase reporter assays detected the luciferase activity of indicated Shh promoter (WT, MUT1, MUT2, and MUT) in LPS-treated MLE-12 cells under p65 overexpression or not. **p < 0.01. n.s. no statistical significance.

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