Fig. 3: METTL3 promotes the glycolysis of CC cells. | Cell Death & Disease

Fig. 3: METTL3 promotes the glycolysis of CC cells.

From: N6-methyladenosine METTL3 promotes cervical cancer tumorigenesis and Warburg effect through YTHDF1/HK2 modification

Fig. 3

a RT-PCR revealed the METTL3 mRNA level in CC cell lines (SiHa, CaSki, C33A, HT-3) and normal cell line (HaCaT). b The knockdown and overexpression of METTL3 were constructed for the functional experiments. c Western blot analysis indicated the knockdown and overexpression efficiency for the transfection. e Glucose uptake analysis, f lactate production analysis, and g ATP level analysis were analyzed. g Extracellular acidification rate (ECAR) profiles were detected for the glycolytic capacity of METTL3 knockdown and METTL3 overexpression. h Xenograft mice assay in vivo demonstrated the tumor volume of cells transfected with METTL3 knockdown. Data are displayed as mean ± standard deviation. **p < 0.01 vs. control. *p < 0.05 vs. control.

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