Fig. 4: MCC950 suppressed the development of RNV, decreased the number of acellular capillaries and reduced the leakage of retinal vessels. | Cell Death & Disease

Fig. 4: MCC950 suppressed the development of RNV, decreased the number of acellular capillaries and reduced the leakage of retinal vessels.

From: Inhibiting the NLRP3 inflammasome with MCC950 ameliorates retinal neovascularization and leakage by reversing the IL-1β/IL-18 activation pattern in an oxygen-induced ischemic retinopathy mouse model

Fig. 4

One eye of OIR mice was injected with MCC950 at 1 μM (b, n = 14 eyes), 10 μM (c, n = 11 eyes), 100 μM (d, n = 14 eyes) or 1 mM (e, n = 14 eyes), and the contralateral eye was injected with PBS (a, n = 14 eyes) at P12. Retinas were stained with FITC-lectin and flat-mounted at P18. Arrowheads point to positive areas. Statistical analysis was performed using the SNK. ***P < 0.001. Retina trypsin digestion was performed to detect the number of acellular capillaries in PBS-treated (g, n = 3 eyes) and MCC950-treated (h, n = 3 eyes) mice. Acellular capillaries were quantified in 7–8 random fields (i) and marked with red arrows. ***P < 0.001. The OIR mice treated with PBS (j, n = 6 eyes) or MCC950 (k, n = 7 eyes) were infused with Evans blue dye and flat-mounted to detect the fluorescence signal. A representative image is shown above. l Quantification of Evans blue leakage was conducted in two groups: PBS group (n = 5 eyes) and MCC950 group (n = 4 eyes). Data are shown as the mean ± SEM and the statistical analyses were performed using two-tailed Student’s t test. *P < 0.05.

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