Fig. 5: MCC950 regulated the expression of angiogenesis-associated molecules in OIR mice. | Cell Death & Disease

Fig. 5: MCC950 regulated the expression of angiogenesis-associated molecules in OIR mice.

From: Inhibiting the NLRP3 inflammasome with MCC950 ameliorates retinal neovascularization and leakage by reversing the IL-1β/IL-18 activation pattern in an oxygen-induced ischemic retinopathy mouse model

Fig. 5

a–g qRT-PCR was performed to quantify the mRNA expression of angiogenic and antiangiogenic genes, such as VEGF, VEGFR2, VEGFR1, MMP2, MMP9, TIMP2 and TIMP1, in the PBS and MCC950 groups (n = 6–8 mice/group). h–j Western blotting was performed to determine the protein expression levels of these factors (n = 3 mice/group). Data are presented as the mean ± SEM from three independent experiments and were analyzed by two-tailed Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001. OIR oxygen-induced ischemic retinopathy mice, P postnatal day.

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