Fig. 6: MCC950 regulated the density of pericytes and the expression of pericyte-related molecules. | Cell Death & Disease

Fig. 6: MCC950 regulated the density of pericytes and the expression of pericyte-related molecules.

From: Inhibiting the NLRP3 inflammasome with MCC950 ameliorates retinal neovascularization and leakage by reversing the IL-1β/IL-18 activation pattern in an oxygen-induced ischemic retinopathy mouse model

Fig. 6

a Immunofluorescence staining for PDGFR-β (a marker of pericytes) and CD31 (a marker of endothelial cells) in PBS-treated and MCC950-treated OIR mice at P18 (n = 3 mice/group). Arrowheads point to positive areas. b–d The mRNA levels of pericyte-related genes, such as PDGF-B, PDGFR-β, and Ang2, in PBS-treated and MCC950-treated OIR mice were detected by qRT-PCR (n = 6–8 mice/group). e, f Protein levels of PDGF-B, PDGFR-β and Ang2 were detected by western blotting (n = 3 mice/group). Data are presented as the mean ± SEM from three independent experiments and were analyzed by two-tailed Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001. OIR oxygen-induced ischemic retinopathy mice, P postnatal day.

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