Fig. 7: Effect of rIL-1β and rIL-18 on the expression of angiogenesis-associated and pericyte-related molecules in HRMVECs and HRMVPCs. | Cell Death & Disease

Fig. 7: Effect of rIL-1β and rIL-18 on the expression of angiogenesis-associated and pericyte-related molecules in HRMVECs and HRMVPCs.

From: Inhibiting the NLRP3 inflammasome with MCC950 ameliorates retinal neovascularization and leakage by reversing the IL-1β/IL-18 activation pattern in an oxygen-induced ischemic retinopathy mouse model

Fig. 7

qRT-PCR analysis of the mRNA expression of angiogenesis-associated molecules such as VEGF, VEGFR2, VEGFR1, MMP2, MMP9, TIMP2, and TIMP1 and pericyte-related molecules such as PDGF-B and Ang2 in HRMVECs (a) treated with PBS, rIL-18 or rIL-1β and VEGF, MMP9, and PDGFR-β in HRMVPCs (b) treated with PBS, rIL-18 or rIL-1β (n = 3 wells/group). Western blot analysis of the expression of the above mentioned molecular proteins in HRMVECs (c, d) and HRMVPCs (e, f) (n = 3 wells/group). Data are presented as the mean ± SEM from three independent experiments and analyzed by two-tailed Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001.

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