Fig. 1: BMP4 Tg expression in Flk-1+ progenitors abrogate maternal diabetes-suppressed BMP4 expression. | Cell Death & Disease

Fig. 1: BMP4 Tg expression in Flk-1+ progenitors abrogate maternal diabetes-suppressed BMP4 expression.

From: Restoring BMP4 expression in vascular endothelial progenitors ameliorates maternal diabetes-induced apoptosis and neural tube defects

Fig. 1

a Diagram of the Bmp4 transgene construct. b Nuclear GFP positive cells (green) in the Flk-1+ progenitors (red) of E8.5 BMP4 Tg embryos. Scale bar = 20 μm. c Levels of Bmp4 mRNA in E8.5 non-BMP4 Tg embryos and BMP4 Tg embryos from nondiabetic (ND) or diabetes mellitus (DM) dams. d BMP4 protein levels in E8.5 non-BMP4 Tg and BMP4 Tg embryos from ND or DM dams. Quantification of relative expression levels of BMP4 proteins was shown in the graphs. e. BMP4 staining (green) in E8.5 non-BMP4 Tg and BMP4 Tg embryos from nondiabetic (ND) or diabetes mellitus (DM) dams. Cell nuclei were stained with DAPI (blue), Scale bar = 30μm. Double immunostaining BMP4 (green) and Flk-1 (red) in E8.5 non-BMP4 Tg and BMP4 Tg yolk sac from nondiabetic (ND) or diabetes mellitus (DM) dams. Cell nuclei were stained with DAPI (blue), Scale bar = 20μm. Quantification of relative expression levels of BMP4 protein expression (normalized by DAPI in the V-shape neuroepithelium and by in the yolk sac) was shown in the graphs. Experiments were repeated three times with embryos from three different dams in each group (n = 3). * indicates significant difference compared to the other groups (P < 0.05) in one-way ANOVA followed by Tukey tests. ND: Nondiabetic; DM: Diabetes Mellitus.

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