Fig. 7: BMP4 Tg expression relieves ER stress and reduces NTD formation. | Cell Death & Disease

Fig. 7: BMP4 Tg expression relieves ER stress and reduces NTD formation.

From: Restoring BMP4 expression in vascular endothelial progenitors ameliorates maternal diabetes-induced apoptosis and neural tube defects

Fig. 7

a–d Protein levels of ER stress markers in E8.5 non-BMP4 Tg and BMP4 Tg embryos from nondiabetic (ND) or diabetes mellitus (DM) dams. Quantification of relative expression levels of phosphorylated IRE1α, PERK, and eIF2α versus total IRE1α, PERK, and eIF2α and CHOP were shown in the graphs. Experiments were performed using three embryos from three different dams per group (n = 3); * indicates significant difference compared to the other groups (P < 0.05) in one-way ANOVA followed by Tukey tests. E: morphology of non-BMP4 Tg and BMP4 Tg embryos from ND or DM dams, bar = 1 mm in the upper panel and bar = 600 μm in the lower panel. Quantification of NTDs was shown in the graph and statistically tested by Fisher exact tests. ND: nondiabetic; DM: diabetes mellitus. F: morphology of WT with NG, WT with HG and WT with HG + recombinant BMP4 treatment embryos, bar = 1 mm. NG: normal glucose (5 mM glucose); HG: high glucose (25 mM glucose).

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