Fig. 2: Overexpression of HOPS enhances NF-κB-driven transcription.
From: HOPS/Tmub1 involvement in the NF-kB-mediated inflammatory response through the modulation of TRAF6
A Transcriptional activity of HOPS. myc-tagged HOPS was cotransfected in HEK-293T cells with constructs containing multiple copies of the responsive elements (RE) NF-κB-RE, E-box-RE, PP-RE, GAL4-RE. After normalization for transfection efficiency using β-galactosidase activity, reporter gene activity was expressed as Relative Luciferase Units (RLU). Activity in control vector transfected cells was set to 1. All the values are the mean ± SD (n = 3). Statistically significant changes are shown. B Effect of HOPS on NF-κB-dependent transcription in HEK-293T. Same as in A, except that vectors expressing the transcription factors (TF) p65, CLOCK/BMAL1 or PPARγ were cotransfected with constructs containing NF-κB-RE, E-box-RE, or PP-RE, respectively, with or without myc-HOPS. C Effect of HOPS on p65 or RelB/p52-mediated transcription in HEK-293T. Same as in A, except that vectors expressing p65 or RelB and p52 were cotransfected with constructs containing NF-κB-RE, with or without myc-HOPS. D Effect of HOPS on NF-κB-induced transactivation of Cox2-promoter in HEK-293T. Same as in B, except that the vector expressing p65 was cotransfected with a construct containing Cox2 full promoter, with or without myc-HOPS. E Effect of HOPS on transactivation of NF-κB-REs induced by p65 or p65/p50 and p65/p52 dimers in HEK-293T. Same as in C, except that vector expressing p65 was transfected alone or cotransfected with p50 (left) or p52 (right), together with NF-κB-RE, with or without myc-HOPS. F NF-κB activation is HOPS-dependent in MEFs. p65 was cotransfected with NF-κB-RE in Hops+/+ and Hops−/− cells.
