Fig. 3: HOPS potentiates p65 target genes transcription.
From: HOPS/Tmub1 involvement in the NF-kB-mediated inflammatory response through the modulation of TRAF6
A mRNA expression of NF-κB-target genes in RAW 264.7 cells transfected with p65 with or without HOPS, measured by qPCR. Values in untransfected cells (not shown) are set to 1. All values are the mean ± SD (n = 3). Statistically significant changes are shown. B ChIP from Hops−/− MEFs cells transfected with myc-HOPS or an empty vector. Cells were collected after 24 h of transfection, subjected to dual cross-link and ChIP with acetyl-H3(K9) antibody or rabbit IgG was performed. Primers for NF-κB sites and 3′ UTR of Il-6 promoter and NF-κB sites and proximal regions of Cox2 promoter were used for qPCR. NF-κB sites binding was detected within Gapdh promoter as negative control. All the values are the mean ± SD (n = 3). Statistically significant changes are shown.
