Fig. 4: HOPS shuttles between the nucleus and the cytoplasm during LPS treatment.
From: HOPS/Tmub1 involvement in the NF-kB-mediated inflammatory response through the modulation of TRAF6
A mRNA expression of Hops and Il-6 in RAW 264.7 cells treated with LPS for the indicated time, measured by qPCR. All the values are the mean ± SD (n = 3). B Protein expression of HOPS in RAW 264.7 cells treated with LPS for the indicated time. α-tubulin was used as loading control. C Nuclear and cytoplasmic extracts prepared from RAW 264.7 cells treated with LPS for the indicated times. WB analysis was performed using anti-HOPS antibody. α-tubulin and lamin B1 were used as loading control for cytoplasm and nuclei, respectively. Graphs on the right show densitometric analysis for HOPS expression levels in cytoplasmic and nuclear fractions. D HOPS immunolocalization in RAW 264.7 cells treated with LPS for the indicated times, analyzed using HOPS antibody (red). Nuclei were DAPI stained (blue). Representative images are shown. Bars, 10 μm. E Percentage of HOPS-positive nuclei observed and counted at the indicated times after LPS treatment. Bars represent mean ± SD (n = 10 field of observation form at least 3 independent samples).
